塑料涂管固相分离方法及应用

• 作 者: 孙贵连 王俊生 何炜 陈昆明
• 出 处: 天津医科大学学报
• 发表日期: 1996年01期
• 关键词: 共聚体 固相放免分析法 共价结合 IgG 涂布 顺丁烯二酸酐 常规液 待测血清 克分子比 温育

本文用化学方法将顺丁烯二酸酐和苯乙烯的共聚体涂布聚苯乙烯塑料试管，使之在管壁上形成一层共聚体薄膜［１］。利用共聚体中的顺丁烯二酸酐能与蛋白质中的氨基迅速结合的特性将抗体（ＩｇＧ）共价结合到管壁上，成功地制备了固相抗体涂布管。并以此建立了甲状腺素（Ｔ４）固相放免分析法。结果表明本方法简便易行。和常规液相放免分析法对比观察无论是标准线、灵敏度、准确度及测定病人样品其结果基本相同。

In this paper, the maleic anhydride and styrene copolymer was coated with a polystyrene plastic tube chemically to form a copolymer film on the tube wall [1]. The solid-phase antibody coated tube was successfully prepared by covalently binding an antibody (IgG) to the tube wall using the ability of the maleic anhydride in the copolymer to rapidly bind to the amino group in the protein. And thus established a thyroxine (T4) solid phase radioimmunoassay. The results show that this method is simple and easy to implement. Compared with the conventional liquid RIA method, the results of the standard line, sensitivity, accuracy and determination of the patient samples are basically the same.