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对含牛粪、玉米秸秆、废菜叶和废纸的农村垃圾进行堆肥实验,实验分为预堆腐和蚯蚓处理2个阶段。预堆腐以EM(Effective Microorganisms)原液和有机物料腐熟剂(FU)为促腐剂,预堆腐结束后,堆料继续进行蚯蚓处理。利用聚合酶链反应与变性梯度凝胶电泳联用技术(PCR-DGGE)分析了对照组(CK)及适合蚯蚓生长繁殖的4‰FU和5‰EM组蚯蚓处理前后细菌和真菌群落中的优势菌群。结果表明:在4‰FU组、5‰EM组和CK组的细菌群落中均存在且占有优势的种群与藤黄单胞菌属和梭状芽孢杆菌属具有较高的亲缘性;在5‰EM组出现的占优势的真菌种群与轮枝孢属具有较高的亲缘性,在CK组中出现的占优势的真菌种群与镰孢菌属和曲霉属具有较高的亲缘性。
The compost experiments on rural garbage containing cow dung, corn stalks, waste vegetable leaves and waste paper were divided into two stages: pre-compost and earthworm treatment. Pre-piled with EM (Effective Microorganisms) stock solution and organic material composting agent (FU) as a preservative, after pre-stacking end, the stack material to continue earthworm treatment. The advantages of the control group (CK) and the 4 ‰ FU and 5 ‰ EM groups suitable for earthworm growth and propagation before and after the earthworm treatment were analyzed by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) Flora. The results showed that there were higher relatives among the 4 ‰ FU group, 5 ‰ EM group and CK group among the bacterial communities, and the dominant populations were related to both genus Romyces and Clostridium. At 5 ‰ The dominant fungi in EM group had higher relatives with Verticillium, and the dominant fungi in CK showed higher phylogenetic relationships with Fusarium and Aspergillus.