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目的采用CdTe纳米量子点为荧光探针,建立一种快速测定环境水样中挥发酚总量的检测方法。方法采用电化学方法合成硫普罗宁表面配位的CdTe荧光量子点,环境水样中的挥发酚对此CdTe量子点具有灵敏的荧光猝灭作用,挥发酚的浓度和量子点荧光强度的降低呈线性相关,根据量子点的荧光猝灭程度来计算水样中挥发酚的浓度。结果在0.01μg/L~0.54μg/L时,所得挥发酚的线性回归方程为y=1.09+5.31x,相关系数(r)=0.997。方法的检出限为0.27 ng/L,平均回收率为92.5%~96.8%,相对标准偏差(RSD)<5%。结论该方法具有快速、重现性好、定量准确和检测灵敏度高等优点,适用于环境水样中痕量挥发酚的测定。
OBJECTIVE To establish a rapid and sensitive method for the determination of the total amount of volatile phenols in environmental water samples using CdTe nanometer quantum dots as fluorescent probe. Methods Electrochemical method was used to synthesize CdTe fluorescent quantum dots with surface coordination of tiopronin. The volatile phenol in environmental water samples had a sensitive fluorescence quenching effect on CdTe quantum dots. The concentration of volatile phenol and the fluorescence intensity of quantum dots were decreased Linear correlation, according to the quantum dot fluorescence quenching level to calculate the concentration of volatile phenol in the water sample. The linear regression equation of the volatile phenol was y = 1.09 + 5.31x and the correlation coefficient (r) was 0.997 when the concentration ranged from 0.01μg / L to 0.54μg / L. The detection limit of the method was 0.27 ng / L, with the average recoveries of 92.5% -96.8% and the relative standard deviations (RSDs) <5%. Conclusion The method is rapid, reproducible, accurate and sensitive. It is suitable for the determination of trace amounts of volatile phenols in environmental water samples.