论文部分内容阅读
蜜环菌(Armillaria mellea)是珍贵药用植物天麻的主要营养供应者,本身也有一定的药用和食用价值,现已在我国大量生产。但蜜环菌在人工培养条件下容易退化,因此,在生产中需要简单易行的复壮方法。本文对此进行了研究,现报道如下: 材料与方法 1、菌种 蜜环菌种引自陕西省微生物研究所。经二年之中数次继代转接,已出现诸如生长变慢、菌索变细,褐色素分泌增多等退化现象。 2、培养基 1号:去皮土豆200克(煮水)、葡萄糖20克,KH_2PO_41.5克,MgSO_4.7H_2O1.5克,蛋白胨5—8克,琼脂18克,水1000毫升。用10％NaOH调PH为
Armillaria mellea is a major nutrient supplier of the precious medicinal plant Gastrodia elata. It also has its own medicinal and edible value, and it is now produced in large quantities in China. However, Armillariella tends to degrade under artificial culture conditions. Therefore, a simple and easy rejuvenation method is required in production. This article has carried on the research to this, report as follows now: Materials and methods 1, strains The species of armillaria is introduced from Shaanxi Institute of Microbiology. After several successive transfers in two years, there have been degenerative phenomena such as slower growth, smaller cords, and increased secretion of brown pigments. 2. Medium No. 1: 200 grams of peeled potatoes (cooked water), 20 grams of glucose, KH 2 PO 41.5 grams, MgSO 4 4.7H 2 O 1.5 grams, peptone 5-8 grams, 18 grams of agar, and 1000 ml of water. Adjust PH with 10% NaOH