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从尼日利亚伊巴丹市3例发热但未经氯喳治疗的病儿(6月～5岁)体内分离得到3份恶性疟原虫分离群,用体外法测定其对氯喹和阿莫地喹的敏感性。实验采用96井微量测定板体外测定方法,将分离得到的感染恶性疟原虫的红细胞悬液(红细胞感染率为0.5～1％)和已配制的不同浓度的药物加至测定板各井内,置蜡烛缸中培养24小时,更换培养液后继续培养24小时,然后从测定板各井中取出红细胞悬液制成薄血膜,常规固定、染
Three isolates of Plasmodium falciparum were isolated from 3 infants (6 months to 5 years old) who were fever but were not treated with clopidogrel in the city of Ibadan, Nigeria. The sensitivity to chloroquine and amodiaquine was determined by in vitro method Sex. Experiments using 96 wells microplate determination method in vitro, the isolated infected erythrocytic suspension of Plasmodium falciparum (erythrocyte infection rate of 0.5 to 1%) and the preparation of different concentrations of drugs added to the assay plate wells, set the candle Cylinder training for 24 hours, after the replacement of culture medium and continue to train for 24 hours, and then remove the erythrocyte suspension from the wells of the assay plate to make a thin blood film, routinely fixed, dyed