论文部分内容阅读
目的及时了解海南省2009年流感样病例及其密切接触者中甲型H1N1流感病毒感染情况。方法应用实时荧光定量PCR法对呼吸道鼻咽拭子标本中的流感病毒核酸进行定性检测。结果全年共检测哨点监测和爆发疫情标本1 148份,荧光定量PCR法检测结果:流感病毒A型核酸阳性为686份,B型核酸阳性为7份。流感样病例中流行型别以A型为主,占59.6%;A型中甲型H1N1流感病毒核酸阳性为500例,占72.9%(500/686),其中学生占84.8%(424/500)。10月-11月份达到流行高峰。随机抽取30份核酸阳性标本进行核酸序列测定,27份为甲型H1N1流感病毒。结论 2009年海南省流感样病例中甲型H1N1流感病毒感染主要以学生为主,开展流感病毒核酸检测,有助于尽早了解流感样病例中流感病毒感染情况,为及时了解甲型H1N1流感疫情的动态变化以及制定科学有效的甲型H1N1流感防控策略提供可靠的实验室依据。
Objective To understand the influenza A (H1N1) virus infection in Hainan Province in 2009 and its close contacts. Methods Real-time fluorescence quantitative PCR was used to qualitatively detect influenza virus nucleic acid in nasopharyngeal swab samples of respiratory tract. Results A total of 1 148 sentinel surveillance and outbreak samples were detected in the whole year. Fluorescence quantitative PCR showed that the positive rate of influenza A virus was 686 and that of type B was 7. The prevalence of influenza-like cases was Type A, which accounted for 59.6%. Type A influenza A (H1N1) virus was positive for nucleic acid in 500 cases (72.9%), of which 84.8% (424/500) . October-November reached the peak of popularity. A total of 30 nucleic acid samples were randomly selected for nucleic acid sequencing. 27 were influenza A (H1N1) viruses. Conclusions Influenza A (H1N1) virus infection in Hainan Province was mainly student-centered in 2009, and influenza virus nucleic acid was detected in the sample of influenza-like cases in Hainan Province. It is helpful to understand the influenza virus infection in influenza-like cases as soon as possible. In order to keep abreast of the epidemic of H1N1 influenza Dynamic changes and the development of a scientific and effective prevention and control strategy of Influenza A (H1N1) influenza to provide a reliable laboratory basis.