论文部分内容阅读
发展了一种基于酶催化金属银沉积信号放大的新型高灵敏气相压电免疫传感检测技术.先将血吸虫抗原(SjAg)共价固定在石英晶体表面,制备得到血吸虫压电免疫传感器.检测时,在晶振上滴加不同浓度的待测血吸虫抗体,再将碱性磷酸酶标记的二抗通过夹心方式结合到传感器表面.然后利用碱性磷酸酶催化磷酸化的抗坏血酸酯水解从而还原硝酸银,使金属银沉积在晶振表面上,放大传感器的质量响应信号.实验结果表明该传感检测方法可显著提高气相压电免疫传感器的检测灵敏度,传感器对血吸虫抗体的响应线性范围在1～225 ng/mL,检测下限为1 ng/mL.
A novel high sensitivity gas-phase piezoelectricity immunosensor detection technology based on enzyme-catalyzed metal-silver deposition signal amplification was developed. Schistosome piezoelectric immunosensor was prepared by covalently immobilizing Schistosoma japonicum antigen (SjAg) on the surface of quartz crystal. , Dropping different concentrations of the tested schistosome antibody on the crystal oscillator, and then binding the alkaline phosphatase labeled secondary antibody to the sensor surface by the sandwich method, then using alkaline phosphatase to catalyze the hydrolysis of phosphorylated ascorbic acid to thereby reduce the silver nitrate, The results show that the sensing method can significantly improve the detection sensitivity of gas-phase piezoelectric immunosensor, and the linear response range of the sensor to schistosome antibody is in the range of 1 ~ 225 ng / mL, the detection limit is 1 ng / mL.