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[目的]为了研究中国石竹叶片愈伤组织的诱导及植株的再生。[方法]通过叶片愈伤组织诱导再分化和叶片直接诱导不定芽2种途径获得了石竹的再生植株。[结果]以植株中部带叶基的叶片愈伤组织诱导再分化效果最好。叶片愈伤组织在MS+6-BA 1.0mg/L+NAA 0.3mg/L培养基中不定芽分化率最高(50%～60%)。幼叶在MS+NAA 0.3mg/L+6-BA1.0mg/L培养基上培养47d可直接诱导出不定芽。与愈伤组织分化成芽相比,幼叶直接诱导不定芽芽数少,但生长快,苗较高。最佳增殖培养基是MS+6-BA 0.2mg/L+IAA 0.2mg/L,最佳生根培养基是1/2MS+IBA 1.0mg/L+NAA 0.1mg/L+0.2%活性碳。[结论]叶片作为外植体具有来源丰富、取材方便、操作简单的优点,探讨植物叶片的再生分化特性具有实际的意义。
[Objective] The research aimed to study the callus induction and plant regeneration of Dianthus chinensis. [Method] The regenerated plants of Dianthus were obtained through inducing re-differentiation of leaf callus and direct induction of adventitious buds by leaves. [Result] The leaf explants with leaves in the middle part of plant had the best effect of inducing redifferentiation. Leaf callus had the highest adventitious bud differentiation rate (50% -60%) in MS + 6-BA 1.0mg / L + NAA 0.3mg / L medium. Adventitious buds were induced directly after young leaves were cultured on MS + NAA 0.3 mg / L + 6-BA 1.0 mg / L for 47 days. Compared with callus differentiation into buds, young leaves directly induced fewer adventitious buds, but grew faster and had higher seedlings. The best medium for growth was MS + 6-BA 0.2 mg / L + IAA 0.2 mg / L. The best rooting medium was 1/2 MS + IBA 1.0 mg / L + NAA 0.1 mg / L + 0.2% activated carbon. [Conclusion] The leaf explants as explants had the advantages of rich source, easy access to materials and simple operation. It is of practical significance to explore the regenerative differentiation of plant leaves.