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冷冻是长期保存细胞的唯一安全方法。一般是用含10％甘油或二甲基亚砜(DMSO)及10％～50％血清的基础培养基来冻存细胞,这些添加剂能保护细胞抵抗细胞内冰晶和渗透效应引起的冷冻损伤。但是,由于在细胞培养中使用血清可导致一些问题,例如每批血清的质量和组成不同,存在着被支原体、病毒等污染的危险性等,所以人们开发了各种实验室及工业用无血清培养基(SFM)。尽管这样,在液氮中保存细胞的培养基一般仍含有血清。为了证明细胞冻存可以在无血清或血清蛋白的情况下进行,作者在最近评估的SFM MDSS2基础上开发了一种SFM以用于液氮中动物细胞的保存。作者选用BHK-21细胞和Vero细胞进行培养和冷冻研究。
Freezing is the only safe method of long-term preservation of cells. Cells are typically stored in basal medium containing 10% glycerol or dimethyl sulfoxide (DMSO) and 10% to 50% serum, which protect cells against freezing damage caused by intracellular ice crystals and osmotic effects. However, since the use of serum in cell culture can lead to problems such as the risk of being contaminated with mycoplasma, viruses and the like depending on the quality and composition of each serum, various kinds of laboratory and industrial serum-free Medium (SFM). In spite of this, the medium in which the cells are kept in liquid nitrogen generally still contains serum. To demonstrate that cell cryopreservation can be performed in the absence of serum or serum proteins, the authors developed an SFM based on the recently evaluated SFM MDSS2 for the preservation of animal cells in liquid nitrogen. The authors selected BHK-21 cells and Vero cells for culture and freezing studies.