论文部分内容阅读
同时采用四种方法,即溶血空斑技术(PFC)、定量溶血分光光度法(QHS)、血凝法(HA)和溶血素法检测了蓖麻毒素对小鼠抗SRBC初次体液免疫应答功能的影响作用。结果表明,这四种检测方法所得的结果基本上是平行的,可互为佐证,均可用于体液免疫应答的检测手段。但从效果来看,PFC法为最好,虽然其百分误差比QHS法要大些,但其灵敏度却比QHS法高近5倍,因此PFC法更能反映免疫应答对剂量的依赖关系及其强弱程度;虽然溶血素法敏感度也高,但其百分误差则更大;血凝法的效果与QHS法相近似,但血凝法操作更简便易行,快速而经济,作为初检手段更有价值。
At the same time, four kinds of methods, hemolytic plaque assay (PFC), quantitative hemolytic spectrophotometry (QHS), hemagglutination (HA) and hemolysin were used to test the anti-SRBC primary humoral immune response Impact. The results show that the results obtained by these four detection methods are basically parallel, which can be mutually supportive and can be used for the detection of humoral immune response. However, from the point of view of effect, the PFC method is the best. Although the percentage error is larger than the QHS method, its sensitivity is nearly 5 times higher than that of the QHS method. Therefore, the PFC method can better reflect the dose dependence of the immune response and its Although the sensitivity of hemolysin method is also high, the percentage error is even greater. The effect of hemagglutination is similar to that of QHS, but hemagglutination is easier, faster and more economical. More valuable.