论文部分内容阅读
目的通过Carba NP试验对革兰阴性菌产生的碳青霉烯酶进行快速检测,了解临床产碳青霉烯酶的革兰阴性菌的临床分布及耐药特征,并验证CarbaNP试验准确性,为临床合理使用抗菌药物提供理论依据。方法选取医院2013年10月-2014年10月检出1 135株革兰阴性菌,利用Carba NP比色法对其中90株多耐药菌产碳青霉烯酶进行检测,并通过改良Hodge试验,亚胺培南-EDTA(乙二胺四乙酸)双纸片协同实验等方法验证其准确性、特异性。结果肺炎克雷伯菌、鲍氏不动杆菌主要来自呼吸道标本,共693株,占61.06%,铜绿假单胞菌主要来自痰液及烧伤创面脓汁,共425株,占37.44%,1 135株革兰阴性菌筛选出多耐药菌为90株,占7.93%,将90株多耐药的菌株经Carba NP法检测出产碳青霉烯酶株22株。结论 Carba NP试验用于检测碳青霉烯酶操作简单、快速、灵敏、费用低廉、特异性强,可有助于提高产碳青霉烯酶革兰阴性菌的检出率和抗菌药物的合理使用水平。
Objective To detect the clinical distribution and drug resistance characteristics of carbapenemase producing carbapenema by Carba NP test and to verify the accuracy of CarbaNP test as Clinical rational use of antimicrobial agents to provide a theoretical basis. Methods A total of 1 135 strains of Gram-negative bacteria were detected in hospitals from October 2013 to October 2014. Carbapenem colorimetric assay was used to detect carbapenemases in 90 of them. The modified Hodge test , Imipenem - EDTA (ethylenediaminetetraacetic acid) double paper synergistic experiments and other methods to verify its accuracy and specificity. Results Klebsiella pneumoniae and Acinetobacter baumannii were mainly from respiratory specimens, accounting for 61.06%, accounting for 61.06%. Pseudomonas aeruginosa mainly came from sputum and burn wound pus juice, with a total of 425 strains (37.44%, 1 135) Strains of Gram-negative bacteria screened out 90 strains of multidrug-resistant bacteria accounting for 7.93%. Twenty-nine strains of multi-drug resistant strains were detected by Carba NP method. Conclusion Carba NP test for the detection of carbapenemases is simple, rapid, sensitive, inexpensive and specific, which can be used to improve the detection rate of carbapenema-producing Gram-negative bacteria and the rational use of antibacterials Use level.