论文部分内容阅读
目的:探讨醒脑静改善高血氨大鼠空间学习记忆功能的分子机制。方法:雄性SD大鼠36只,随机分为3组,每组12只:正常对照组(A组),高血氨模型组(B组),醒脑静治疗组(C组),Morris水迷宫观察动物空间学习记忆的变化。大鼠处死后,检测血氨,RT-qPCR检测大鼠脑组织CREB(cAMP应答元件结合蛋白,cAMP-response element binding protein)基因的表达,western blot检测脑组织磷酸化CREB蛋白的表达。结果:与正常对照组比较,高血氨模型组及醒脑静治疗组大鼠血氨水平明显升高。与高血氨模型组相比,醒脑静治疗组大鼠寻找障台的平均逃避潜伏期、游泳总距离均缩短。与正常组比较,高血氨模型组大鼠皮层与海马组织CREB基因mRNA和蛋白表达均明显下降。醒脑静治疗组大鼠皮层与海马CREB基因mRNA和蛋白表达,较高血氨模型组均明显升高。结论:醒脑静在一定程度上改善了大鼠的空间学习记忆能力,其影响脑内CREB表达可能是其发挥作用的重要途径。
Objective: To investigate the molecular mechanism of Xingnaojing in improving spatial learning and memory in hypernathalmic rats. Methods: Thirty - six male Sprague - Dawley rats were randomly divided into 3 groups (n = 12): normal control group (group A), high ammonia group (group B), Xingnaojing group (group C) Maze observation of animal spatial learning and memory changes. After sacrificed, blood ammonia was assayed for the expression of CREB (cAMP-response element binding protein) gene in rat brain by RT-qPCR. The phosphorylation of CREB protein was detected by western blot. Results: Compared with the normal control group, the levels of serum ammonia in the model group and Xingnaojing group were significantly increased. Compared with hyperammonemia model group, the average escape latency of looking for barrier in Xingnaojing group was shorter and the total swimming distance was shorter. Compared with the normal group, CREB gene mRNA and protein expression in the cortex and hippocampus of the model rats of high blood ammonia decreased significantly. Xingnaojing treatment group rats cortex and hippocampal CREB gene mRNA and protein expression, higher ammonia group were significantly higher. Conclusion: Xingnaojing can improve spatial learning and memory in rats to a certain extent. Its effect on the expression of CREB in the brain may be an important way to play its role.