大肠癌端粒酶测定及其临床生物学意义研究的初步报告

• 作 者: 万德森 刘宗石 吴珊 潘志忠 周志伟 张桥 陈雯
• 机 构: 中山医科大学肿瘤医院腹科,香港中文大学肝肠胃病理科,中山医科大学卫生学院毒理学教研室
• 出 处: 癌症
• 发表日期: 1998年01期
• 关键词: 临床生物学 大肠癌端粒酶 大肠肿瘤 大肠癌组织 正常大肠组织 意义研究 分化程度 检测阳性率 早期大肠癌 病理分类

目的：为了探索端粒酶（ＴＬＭＡ）与大肠癌的关系。方法：采用Ｋｉｍ等建立的端粒重复序列扩增法（ＴＲＡＰ）测定３２例大肠癌组织和正常大肠组织ＴＬＭＡ活性。结果：３２例大肠癌组织ＴＬＭＡ强阳性１１例，弱阳性６例，阴性１５例，阳性率５３．１％。３２例正常大肠组织ＴＬＭＡ弱阳性２例（６．３％），余均为阴性。结论：大肠癌组织ＴＬＭＡ活性检测阳性率高，与性别、年龄、部位、病理分类、侵袭范围、分期等无明显关系，提示有可能成为早期发现指标之一。

Objective: To explore the relationship between telomerase (TLMA) and colorectal cancer. METHODS: The telomere repeat sequence amplification (TRAP) method established by Kim et al. was used to determine the TLMA activity in 32 cases of colorectal cancer tissues and normal colorectal tissues. RESULTS: Thirty-one patients with colorectal cancer had positive TLMA in 11 cases, weakly positive in 6 cases, and negative in 15 cases. The positive rate was 53.1%. Thirty-two cases (6.3%) of TLMA were weakly positive in 32 cases of normal colorectal tissue. The rest were negative. Conclusion: The detection rate of TLMA activity in colorectal cancer tissue is high, and it has no obvious relationship with gender, age, location, pathological classification, invasion scope, staging, etc., suggesting that it may be one of the early detection indicators.