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目的:考察板蓝根不同提取部位的体外抗氧化活性。方法:用大孔树脂和阴、阳离子交换树脂制备板蓝根3个有效部位:总木脂素、总有机酸、总生物碱部位。结合酶标仪,通过清除1,1-二苯基苦基苯肼(DPPH)自由基和Fe3+还原能力(FRAP)测定板蓝根提取物的体外抗氧化作用。结果:板蓝根颗粒组及各个提取物组均有不同程度的体外抗氧化效果。其中以总木脂素抗氧化效果最佳(DPPH IC50160.6 mg·L-1;FRAP 0.75 mmol·g-1);其次为板蓝根颗粒组(DPPH IC50595.97 mg·L-1;FRAP 0.12 mmol·g-1);总有机酸,总生物碱部位效果较弱。结论:测定了板蓝根中不同有效部位的抗氧化活性,初步确定其抗氧化相关活性成分主要存在于木脂素部位。
Objective: To investigate the antioxidant activity of Radix isatidis in different extraction sites. Methods: Three active sites of Radix Isatidis were prepared with macroporous resin, anion exchange resin and cation exchange resin: total lignan, total organic acids and total alkaloid. Combined with microplate reader, the anti-oxidative activity of Radix Isatidis in vitro was determined by removing DPPH and Fe (3) reduction ability (FRAP). Results: Banlangen Granules group and each extract group have different degrees of in vitro antioxidant effect. Among them, the antioxidant activity of total lignans was the best (DPPH IC50160.6 mg · L-1; FRAP 0.75 mmol · g-1), followed by Banlangen granules group (DPPH IC50595.97 mg · L-1; FRAP 0.12 mmol · G-1); total organic acids, total alkaloid site effect is weak. CONCLUSION: The antioxidant activities of different effective parts of Radix isatidis were determined, and the active components related to antioxidation were primarily identified in lignans.