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目的 在体条件下观察 β-淀粉样蛋白 ( Aβ)对白细胞介素 -1 β( IL-1 β)和肿瘤坏死因子 -α( TNF-α)表达的影响 ,探讨它们在 Alzheimer病 ( AD)发病机制中的作用。方法 以不同浓度 Aβ溶液及生理盐水作海马立体定向注射后 ,采用免疫组织化学、HE染色及刚果红染色方法 ,显示胶质细胞反应及 IL-1β、TNF-α表达情况 ;消炎痛灌胃治疗模型大鼠并以蒸馏水作治疗对照。采用方差分析对量化指标行统计学处理。结果 胶质细胞反应程度及 IL-1 β、TNF-α的表达水平随 Aβ浓度增大而增加 ,4 μg/ μL Aβ溶液注射组 IL-1 β、TNF-α阳性细胞数 (个 /视野 )分别为 4 1 .75± 2 .6 1和 5 2 .1 7± 5 .2 3,显著多于生理盐水注射组 ( 2 1 .1 6± 2 .92和 31 .0 7± 3.5 8,P<0 .0 1 ) ;消炎痛显著降低 IL-1 β、TNF-α的表达水平。结论 在体条件下高浓度 Aβ能诱导胶质细胞 IL-1 β和 TNF-α的表达 ,并可被消炎痛抑制 ,结果提示炎症反应与 AD的发病机制可能有密切关系。
Objective To observe the effect of β-amyloid protein (Aβ) on the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF- The role of pathogenesis. Methods The hippocampus was injected stereotaxially with different concentrations of Aβ solution and normal saline. Immunohistochemistry, HE staining and Congo red staining were used to observe glial cell responses and the expression of IL-1β and TNF-α. Indomethacin treatment Model rats were treated with distilled water as a control. Analysis of variance using the statistical indicators of statistical processing. Results The glial cell response and the expression of IL-1β and TNF-α increased with the increase of Aβ concentration. The number of IL-1β and TNF-α-positive cells in 4 μg / μL Aβ injection group Respectively, 4 1 .75 ± 2. 6 1 and 5 2 .1 7 ± 5 .2 3, significantly more than the saline injection group (21.162 ± 2.92 and 31.073 ± 3.58, P <0. 01). Indomethacin significantly decreased the expression of IL-1β and TNF-α. Conclusions High concentrations of Aβ can induce the expression of IL-1β and TNF-α in glial cells under in vivo conditions and can be inhibited by indomethacin. The results suggest that the inflammatory response may be closely related to the pathogenesis of AD.