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目的:研究明党参的遗传多样性,为明党参种质资源利用提供理论依据。方法:以10个不同居群的明党参为材料,通过SRAP分析,计算各样品间的遗传相似系数,在此基础上采用UPGMA方法进行聚类,构建树状图。结果:从160个引物组合中筛选得到17个多态性引物组合,共检测到363个基因位点,其中多态性位点有314个,平均每个引物组合产生18.47个多态性位点,多态性位点百分率为86.50%,显示了较高的多态性比率;各居群遗传相似系数在0.495 9~0.818 2;根据聚类结果可将10个不同居群的明党参分为两大类。结论:明党参不同居群间具有高度的遗传多样性;不同居群的亲缘关系与其地理分布有一定的相关性。
Objective: To study the genetic diversity of Codonopsis pilosula and provide the theoretical basis for the utilization of Codonopsis pilosula. Methods: The genetic similarity coefficient of each sample was calculated by SRAP analysis in 10 different populations of C. catechu. Based on this, the UPGMA method was used to cluster and construct the dendrogram. Results: A total of 17 polymorphic primer combinations were screened from 160 primer combinations. A total of 363 gene loci were detected, of which 314 were polymorphic, with an average of 18.47 polymorphic loci per primer combination , The percentage of polymorphic loci was 86.50%, showing a high polymorphism rate; the genetic similarity coefficient of each population was between 0.495 9 and 0.818 2; According to the clustering results, Two major categories. Conclusion: There is a high degree of genetic diversity among different populations of C. cumingii. The genetic relationship among different populations is related to their geographical distribution.