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目的:探讨维生素D受体(vitaminDreceptor,VDR)基因多态性与中国湖南省汉族人群脊柱结核易感性的关系。方法:选取2004年10月至2006年2月我院收治的湖南省汉族新发脊柱结核患者42例(病例组)及志愿者64例(对照组),应用聚合酶链反应-限制性片断长度多态性分析技术检测两组对象VDR基因FokI酶切位点多态性,并进行VDR基因分型。结果:VDR-FF、VDR-Ff与VDR-ff三种基因型在病例组和对照组中的分布频率分别为14.29%、35.71%、50%和23.45%、54.69%、21.86%;两组组间比较有显著性差异(P<0.05),其中VDR-ff基因型在病例组中的分布频率明显高于对照组,比值比(oddsratio,OR)为3.571(P<0.05),其95%可信区间(confidenceinterval,CI)为1.561～8.167。结论:VDR基因FokI酶切位点多态性与湖南省汉族人群脊柱结核的易感性相关,VDR-ff基因型可能是其易感基因型。
Objective: To investigate the relationship between vitamin D receptor (VDR) gene polymorphism and susceptibility to spinal tuberculosis in Han nationality in Hunan province, China. Methods: Totally 42 cases (cases group) and 64 volunteers (control group) were enrolled from our hospital from October 2004 to February 2006 in Hunan province. Polymerase chain reaction-restriction fragment length Polymorphism analysis was used to detect FokI polymorphism of VDR gene in both groups and VDR genotyping was performed. Results: The frequencies of VDR-FF, VDR-Ff and VDR-ff were 14.29%, 35.71%, 50% and 23.45%, 54.69% and 21.86% respectively in the case group and the control group. (P <0.05). The distribution frequency of VDR-ff genotype in case group was significantly higher than that in control group, odds ratio (OR) was 3.571 (P <0.05), 95% The confidence interval (CI) was 1.561 ~ 8.167. CONCLUSION: VDR gene FokI restriction site polymorphism is associated with susceptibility to spinal tuberculosis in Han population in Hunan Province. VDR-ff genotype may be the susceptible genotype.