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Objective To explore the influence of galectin-3 on missed abortion.Methods Forty cases of normal intrauterine early pregnancy were randomly divided into 2 groups: surgical abortion group(group A, n=20) and medical abortion group(group B, n=20). The third group was missed abortion group(group C, n=20) with the gestational age less than 13 weeks. Serum was isolated from the blood samples,collected and used for ELISA quantification of galectin-3. Villus and decidua tissues were collected from the abortus for immunohistochemical examination and real-time fluorescence relative quantitative PCR.Results The level of galectin-3 in the serum was the lowest in missed abortion group(P<0.05). Immunohistochemistry showed that galectin-3 expression in villus of missed abortion group was significantly lower than that of surgical abortion group(P<0.01).Real-time fluorescence relative quantitative PCR showed that galectin-3 m RNA relative expression in villus of missed abortion group(2-△△Ct=0.04±0.01) was significantly lower than that of surgical abortion group(2-△△Ct=1.00±0.00). Galectin-3mR NA relative expression in deciduas of medical abortion group(2-△△Ct=0.08± 0.02)was significantly lower than that of surgical abortion group(2-△△Ct=1.00±0.00)(P<0.01).Conclusion Galectin-3 is related to the development of villus and decidua during early pregnancy. The decreased expression of galectin-3 may promote the occurrence of missed abortion.
Objective To explore the influence of galectin-3 on missed abortion. Methods Forty cases of normal intrauterine early pregnancy were randomly divided into 2 groups: surgical abortion group (group A, n = 20) and medical abortion group (group B, n = 20 The third group was missed abortion group (group C, n = 20) with the gestational age less than 13 weeks. Serum was isolated from the blood samples, collected and used for ELISA quantification of galectin-3. Villus and decidua tissues were collected from the abortus for immunohistochemical examination and real-time fluorescence relative quantitative PCR. Results of the level of galectin-3 in the serum was the lowest in missed abortion group (P <0.05). Immunohistochemistry showed that galectin-3 expression in villus of missed Real-time fluorescence relative quantitative PCR showed that galectin-3 m RNA relative expression in villus of missed abortion group (2- △△ Ct = 0.04 ± 0.01) was significantly lower than that of surgical abortion group (2- △△ Ct = 1.00 ± 0.00). Galectin-3mR NA relative expression in deciduas of medical abortion group (2- △△ Ct = 0.08 ± 0.02) was significantly lower than than that of the surgical abortion group (2- △△ Ct = 1.00 ± 0.00) (P <0.01) .Conclusion Galectin-3 is related to the development of villus and decidua during early pregnancy. The decreased expression of galectin-3 may promote the occurrence of the occurrence of missed abortion.