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为探讨人重组γ－干扰素（ｈｒＩＦＮ－γ）能否抑制抗原引起嗜酸性粒细胞（ＥＯＳ）对过敏性支气管哮喘患者气道的浸润，选择１０例过敏性哮喘患者以纤支镜直接将ｈｒＩＦＮ－γ及过敏原注入左舌肺叶或右中肺叶的段支气管，将生理盐水及过敏原注入对侧肺相应的段支气管作为对照。２４ｈ后复以纤支镜取出支气管粘膜组织，然后以免疫组化技术探测ＥＯＳ、ＣＤ＋４、ＣＤ＋８以及ＣＤ＋２５淋巴细胞的数量。结果发现ＩＦＮ－γ组支气管粘膜组织中ＥＯＳ总数、ＣＤ＋４及ＣＤ＋２５细胞数明显低于对照组，而ＣＤ＋８数则无明显变化。说明ｈｒＩ－ＦＮ－γ局部用药通过抑制ＣＤ＋４细胞的浸润及Ｔ细胞的活化从而抑制了抗原引起哮喘气道ＥＯＳ的浸润。
In order to investigate whether recombinant human interferon-gamma (IFN-γ) can inhibit the eosinophil (EOS) -induced airway infiltration induced by antigen in allergic bronchial asthma patients, 10 patients with allergic asthma were selected to receive hrIFN -γ and allergens were injected into the segment of left bronchus or right middle lobe of the bronchus, saline and allergen into the contralateral lung corresponding bronchus as a control. Bronchial mucosa tissues were removed with fiberoptic bronchoscopy 24 hours later, and the number of EOS, CD + 4, CD + 8 and CD + 25 lymphocytes was detected by immunohistochemistry. The results showed that the total number of EOS, the number of CD + 4 and CD + 25 cells in the bronchial mucosa of IFN-γ group was significantly lower than that of the control group, while the number of CD + 8 did not change significantly. The results showed that local administration of hrI-FN-γ inhibited the infiltration of EOS in asthmatic airways by inhibiting the infiltration of CD + 4 cells and the activation of T cells.