Primary culture and identify of cerebral cortical astrocytes from Sprague-Dawley rats

• 作 者: ZHANG Hai-wei WU Chun-yang ZHA
• 机 构: DepartmentofPhrmacy,HebeiUniversityofScienceandTechnology
• 出 处: 中国药理学与毒理学杂志
• 发表日期: 2017年5期

OBJECTIVE To establish a method for primary cultured and iden-tified Sprague-Dawley(SD) rats cerebral cortical astrocytes. METHODS Cerebral co-rtex of SD neonatal rats within 24 h was taken with stereo microscope and was cut topieces(1 mm~3),digested by A