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利用蒽环类抗癌药物阿霉素的自发荧光可反映药物在细胞中的分布和相对剂量；利用特异性荧光探针Hoechst33342(Ho．33342）可标记活细胞DNA，从而反映细胞所处周期时相及染色体的形态变化．但是在阿霉素和Ho．33342共存在的细胞中，用MPVⅡ型显微荧光分光光度计对药物的自发荧光和Ho．3342的发射荧光进行光谱分析的结果表明：在紫外激发下，药物自发荧光与Ho.33342荧光之间存在干扰；选择适合的外加带通滤片S525后，这种干扰可降低到可以忽略的程度，从而在同一细胞内可分离开2种荧光，并进行定量分析．该结论有利于今后发展影像分析和荧光多参数相关测量方法，可以通过荧光图像的获取及分析来研究单个活细胞摄取、滞留抗癌药物与细胞增殖、细胞凋亡及抗药性的关系，从而揭示药物的作用机理．
Using the anthracycline anticancer drug adriamycin autofluorescence can reflect the drug distribution in the cells and the relative dose; using a specific fluorescent probe Hoechst33342 (Ho.33342) can be labeled with live cell DNA, which reflects the cell cycle Phase and chromosome morphology changes. But in doxorubicin and Ho. 33342 coexisting cells, the autofluorescence and Ho of the drug were measured using an MPV II microplasma spectrophotometer. 3342 emission fluorescence spectrum analysis showed that: under UV excitation, there is interference between drug autofluorescence and Ho.33342 fluorescence; choose the appropriate external bandpass filter S525, this interference can be reduced to a negligible extent , Which can be separated in the same cell two kinds of fluorescence, and quantitative analysis. The conclusion is conducive to the future development of image analysis and fluorescence multi-parameter correlation measurement method, through the fluorescence image acquisition and analysis to study the single living cell uptake, retention of anti-cancer drugs and cell proliferation, apoptosis and drug resistance, revealing The mechanism of action of drugs.