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目的 探讨我国部分地区嗜肺巴斯德杆菌分子流行病学特征。方法 随机多态扩增PCR(RAPD -PCR) ,两条单一随机引物S1和S3 ,对分离来自北京和南京不同实验动物饲养单位的大鼠、小鼠、野鼠的 1 5株嗜肺巴斯德杆菌及参考株共 1 6株菌基因组随机扩增 ,比较DNA多态性图谱。结果 1 5个流行株可分成 4种基因型 :来自相同饲养单位的小鼠株、大鼠株及野鼠株基因型亦不相同 ;野鼠株与部分小鼠分离株基因型相同。结论 同一地区嗜肺巴斯德杆菌流行株呈明显的多态性 ;野鼠有可能成为实验动物嗜肺巴斯德杆菌的传染源
Objective To investigate the molecular epidemiological characteristics of Pneumococcus pneumoniae in some areas in China. Methods Random amplified polymorphic DNA (RAPD-PCR) and two single random primers S1 and S3 were used to isolate 15 strains of Pneumoconiosis Pasteur from rats, mice and voles of different experimental animals in Beijing and Nanjing A total of 16 strains of bacteria and reference strains of genomic DNA were randomly amplified to compare DNA polymorphism. Results Fifteen popular strains were divided into four genotypes. The genotypes of mouse strains and wild rodents from the same feeding unit were also different. The genotypes of wild rodents and some of the mice were the same. CONCLUSION: The prevalence of Pasteurella multocida pneumoniae in the same area is obviously polymorphic. Vole may become the source of infection of Pasteurella multocida in laboratory animals