基于一步电沉积层状甘氨酸-N,N-双甲基膦酸锆/海藻酸钠/血红蛋白的生物传感器的研究(英文)

• 作 者: 徐静 刘昌华 滕瑛丽 午海霞 吴宗方
• 机 构: 西南大学化学化工学院,
• 出 处: 西南师范大学学报(自然科学版)
• 发表日期: 2010年06期
• 关键词: 生物传感器 N 双甲基 膦酸 电沉积 biosensors 有机-无机杂化 电子转移 实验条件 响应电流

一种新的有机-无机杂化层状甘氨酸-N,N-双甲基膦酸锆被合成并成功的用于固定血红蛋白.层状甘氨酸-N,N-双甲基膦酸锆/海藻酸钠/血红蛋白成功的电沉积到金电极的表面.血红蛋白的电子转移系(α)和电子转移速率常数(ks)分别为0.91和0.647 s-1,表明了血红蛋白酶和金电极之间的电子转移速率较快.在优化的实验条件下,响应电流与过氧化氢的浓度在1.4μmol/L to 3.5 mmol/L范围内呈良好的线性关系,检出限为4.7×10-7mol/L(S/N=3),传感器具有好的灵敏度、重现性、稳定性.

A Novel Organic-Inorganic Hybrid Layered Zirconium Glycine-N, N-Bismethylphosphonate was synthesized and successfully used to immobilize hemoglobin. Layered glycine-N, N-bismethylphosphonate zirconium / alginate Sodium / hemoglobin was successfully electrodeposited onto the gold electrode surface.The electron transfer (α) and electron transfer rate constants (ks) of hemoglobin were 0.91 and 0.647 s-1, respectively, indicating the electron transfer between hemoglobin and gold electrode Under the optimal experimental conditions, the response current and hydrogen peroxide concentration in the range of 1.4μmol / L to 3.5mmol / L showed a good linear relationship with a detection limit of 4.7 × 10-7mol / L (S / N = 3), the sensor has good sensitivity, reproducibility and stability.