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目的观察透明质酸（ｈｙａｌｕｒｏｎｉｃａｃｉｄ，ＨＡ）刺激活性物（ＨＡ－ｓｔｉｍｕｌａｔｉｎｇａｃｔｉｖｉｔｙ，ＨＡＳＡ）对培养的人视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉａｌ，ＲＰＥ）细胞的作用。方法将不同质量浓度的ＨＡＳＡ加入ＲＰＥ细胞培养液，采用活细胞计数、四甲基偶氮唑盐（ｔｅｔｒａｚｏｌｉｕｍ，ＭＴＴ）比色法及氚标胸腺嘧啶核苷（３Ｈ－ｔｈｙｍｉｄｉｎｅ，３Ｈ－ＴｄＲ）掺入法检测细胞活力，用流式细胞仪（ｆｌｏｗｃｙｔｏｍｅｔｒｙ，ＦＣＭ）分析ＲＰＥ细胞周期。结果ＨＡＳＡ在１２．５～２００．０μｇ／ｍｌ范围及４８小时内对人ＲＰＥ细胞的抑制存在剂量－效应及时间－效应关系。最大细胞抑制率约４８．０％。ＦＣＭ显示ＨＡＳＡ作用后Ｇ１期细胞数较对照组增加７．２％，Ｓ期减少９．７％。结论ＨＡＳＡ在一定时间和剂量范围内对人ＲＰＥ细胞有抑制作用。
Objective To observe the effect of hyaluronic acid (HA) stimulating activity (HASA) on cultured human retinal pigment epithelium (RPE) cells. Methods Different concentrations of HASA were added into RPE cell culture medium. The viable cell count, tetrazolium (MTT) colorimetric method and 3H-thymidine (3H-TdR) Cell viability was assayed by flow cytometry, and the cell cycle of RPE was analyzed by flow cytometry (FCM). Results There was dose-effect and time-effect relationship between HASA and human RPE cells in the range of 12.5 ~ 200.0μg / ml within 48 hours. The maximum cell inhibition rate was about 48.0%. FCM showed that the number of cells in G1 phase after HASA treatment increased by 7.2% compared with the control group, and the S phase decreased by 9.7%. Conclusion HASA can inhibit human RPE cells in a certain time and dose range.