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探讨 、 、 型胶原基因表达与软骨性肿瘤分化的关系。应用免疫组织化学和原位杂交技术检测存档石蜡标本软骨瘤、骨软骨瘤、软骨肉瘤和正常软骨共 71例中 、 、 型胶原基因的表达情况。结果显示 :免疫组化检测 型胶原蛋白的阳性率和表达强度 (灰度 ,其数值与染色强度呈反比 ) ,软骨瘤 (10 0 % ,148.99± 14.2 5 )和骨软骨瘤 (95 % ,148.76± 2 1.2 2 )与正常软骨 (10 0 % ,144 .88± 5 .0 5 )相似 ,软骨肉瘤 (74.0 7% ,16 6 .46± 17.6 7)明显低于良性软骨性肿瘤 (软骨瘤和骨软骨瘤 ) (P<0 .0 1) ,而且软骨肉瘤随着分化程度降低 ,阳性率和表达强度逐渐降低 ;高分化软骨肉瘤为 10 0 % (12 /12 ) ,148.14± 16 .10 ;中分化软骨肉瘤为 83.33% (5 /6 ) ,16 8.6 8± 11.82 ;低分化软骨肉瘤则完全不表达 (0 /9) (P <0 .0 5 )。正常软骨没有 、 型胶原 ,良性软骨性肿瘤出现少量 、 型胶原 ,软骨瘤 、 型胶原蛋白的阳性率分别为 38.89%和 44 .44 % ,骨软骨瘤分别为 35 %和 40 %。软骨肉瘤 、 型胶原蛋白明显增多 ,阳性率分别为 77.78%和 88.89% (P<0 .0 1) ,而且随着软骨肉瘤分化程度降低 , 、 型胶原蛋白表达强度增强 (P<0 .0 5 )。原位杂交显示软骨肉瘤 型胶原 m RNA的阳性率 (6 8.75 % ,11/16 )?
To explore the relationship between collagen gene expression and cartilage tumor differentiation. Immunohistochemical and in situ hybridization techniques were used to detect the expression of collagen genes in paraffin-embedded paraffin-embedded cartilage, osteochondroma, chondrosarcoma, and normal cartilage in 71 cases. The results showed: the positive rate and expression intensity of immunohistochemically detected collagen (gray scale, its value is inversely proportional to staining intensity), cartilage (100%, 148.99± 14.25) and osteochondroma (95%, 148.76) ± 2 1.2 2) Similar to normal cartilage (100%, 144.88±5.05), chondrosarcoma (74.07%, 166.66±17.67%) was significantly lower than benign cartilage tumors (chondroma and Osteochondroma) (P < 0.01), and chondrosarcoma decreased gradually with the degree of differentiation, and the positive rate and expression intensity gradually decreased; hyperdifferentiated chondrosarcoma was 10% (12/12), 148.14 ± 16.10; The differentially differentiated chondrosarcomas were 83.33% (5 / 6) and 16 8.6 8 ± 11.82; the poorly differentiated chondrosarcomas were not expressed at all (0 / 9) (P <0.05). Normal cartilage did not have collagen and collagen, benign cartilage tumors showed a small amount of collagen, the positive rates of chondroma and collagen were 38.89% and 44.44%, and osteochondroma was 35% and 40%, respectively. The chondrosarcoma and type of collagen increased significantly, the positive rates were 77.78% and 88.89% respectively (P<0.01), and the expression intensity of type-collagen increased as the degree of differentiation of chondrosarcoma decreased (P<0.05). ). In situ hybridization showed a positive rate of chondrosarcoma collagen m RNA (68.75%, 11/16)?