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目的:通过研究过氧化物酶体增生物激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)激动剂吡格列酮对前脂肪细胞(3T3-L1)分化过程中盐皮质激素受体(mineralocorticoid receptor,MR)表达的影响,探讨PPARγ在肥胖及胰岛素抵抗中的作用机制。方法:用吡格列酮对小鼠前脂肪细胞3T3-L1进行刺激,利用实时定量PCR,检测在前脂肪细胞分化为成熟脂肪细胞的过程中,盐皮质激素受体及其相关基因的mRNA表达量。结果:前脂肪细胞分化过程中,给予吡格列酮刺激后细胞分化效率明显增加的同时,盐皮质激素受体及11β-羟化类固醇脱氢酶1(11β-hydroxysteroid dehydrogenase type1,11β-HSD1)的表达量增加。结论:PPARγ在促进前脂肪细胞的分化的同时,明显增加盐皮质激素受体的表达。
OBJECTIVE: To investigate the effects of peroxisome proliferator-activated receptorγ (PPARγ) agonist pioglitazone on mineralocorticoid receptor (MR) in differentiation of preadipocytes (3T3-L1) Expression of the impact of PPARγ in the mechanism of obesity and insulin resistance. Methods: 3T3-L1 preadipocytes were stimulated with pioglitazone. The mRNA expression of mineralocorticoid receptor and its related genes during the differentiation of preadipocytes into mature adipocytes was detected by real-time quantitative PCR. Results: The expression of 11β-hydroxysteroid dehydrogenase type 1 and 11β-HSD1 in the process of preadipocyte differentiation was significantly increased after the cells were treated with pioglitazone. increase. Conclusion: PPARγ can promote the differentiation of preadipocytes and increase the expression of mineralocorticoid receptor.