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采用单因素试验和响应面分析对球孢白僵菌YS03菌株进行产孢培养基的配方改良。单因素试验筛选出对产孢量影响较大的3种原料为花生、麦麸和玉米粉,进而根据Box-Behnken的中心组合原理进行三因素三水平的实验设计,最终得出使产孢量最大的配方为花生∶麦麸∶玉米粉=2.6∶3.5∶3.9。经验证,预测值与验证试验平均值接近,且新配方的产孢量是PDA培养基产孢量的1.92倍。
Single factor experiments and response surface analysis of Beauveria bassiana YS03 spore culture medium formulations improved. The single factor test screened the spore yield of the three major raw materials for the peanut, wheat bran and corn flour, and then based on the principle of Box-Behnken combination of three factors and three levels of experimental design, the final result of sporulation The largest formula is peanut: wheat bran: corn flour = 2.6: 3.5: 3.9. Proved that the predicted value is close to the average value of the validation test, and the sporulation amount of the new formula is 1.92 times of that of the PDA medium.