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目的:探讨CD44vmRNA拼接和异常表达与胃癌临床病理之间的相关意义.方法:应用RT－PCR检测方法对正常胃粘膜、癌旁组织及癌组织中的CD44vmRNA的表达进行研究.对20例患者的60份胃组织标本(20份胃癌组织,20份癌旁组织.20份正常胃粘膜)中CD44v变异表达产物进行检测.结果:有CD44vmRNA阳性表达着胃癌组织16例,癌旁组织5例;16例有淋巴结转移者15例阳性,4倒无淋巴结转移者1例阳性(P＜0.05).5例有远处转移者均为阳性.所有60份胃癌组织、癌旁组织、正常粘膜都表达标准型CD44(CD44s);而且两型胃癌中CD44v表达有差异(P＜0.05),高分化腺癌(肠型)5例中2例阳性,低分化癌(弥漫型)15例中14例阳性.结论:CD44vmRNA在胃癌中的表达有普遍性,既可能是早期癌变出现的生物学指标,也可能是判断胃癌转移的指标.“,”Aim: To study the correlation of rhe CD44 gene splice and abnormal expression with theclinical - pathologic changes of gastric carcinoma. Methods: We detected the expressi0n of CD44VmRNAin the normal gastric mucosa, cancer and it\'s adjacent tissues by RT - PCR. ResultS: CD44vmRNA wasespressed in l6 of 20 gastric cancers, in 5 of 20 the adjacent tissues but was n0t expressed in a1l the 2O nor-mal mucosa specimens; and l5 out of 16 had lymph node metastasis but 1 out 0f 4 had no lymph nodemetastasis, all the five cases investigated showed distant metastasis (p < 0,05). A1l the 6O gastric mu-cosa, gastric cancer and it\'s adjacent tissue presented the standard CD44smRNA. There were differencesin the expression between two types of gastric mucosa (p < O.05), 2 out of 5 were well - differentiatedor intestinal type gastric cancers, 14 out of 15 were Poorly - differentiated 0r diffuse type cancers. Conclu-sion: The abnormal expression of CD44v is of universal significance in gastric cancer which might by mark-ers of gastric cancer and the metastasis.