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[Objective] The aim was to establish effective method for endogenous hormone extraction and explore conditions of chromatographic analysis for three endogenous hormones in rhizome of Alhagi sparsifolia. [Method] Activol (GA3), zeatin (ZR) and indole-3-acetic acid (IAA) in rhizome were separated and measured as per RP-HPLC. [Result] The average recovery rates of GA3, ZR and IAA were 98.3%, 90.3% and 101.3%, respectively, indicating that the method is suitable for quantitative analysis with little errors. The chromatographic conditions were as follows: methanol/0.75% of acetic acid at 45∶ 55 (mobile phase); flow speed at 0.7 ml/min; wavelength at 254 nm; column temperature at 25 °C. [Conclusion] The research preliminarily established HPLC conditions for separation of endogenous hormones in rhizome of Alhagi sparsifolia.
[Objective] The aim was to establish effective method for endogenous hormone extraction and explore conditions of chromatographic analysis for three endogenous hormones in rhizome of Alhagi sparsifolia. [Method] Activol (GA3), zeatin (ZR) and indole-3-acetic acid IAA) in rhizome were separated and measured as per RP-HPLC. [Result] The average recovery rates of GA3, ZR and IAA were 98.3%, 90.3% and 101.3%, respectively, indicating that the method is suitable for quantitative analysis with little The chromatographic conditions were as follows: methanol / 0.75% of acetic acid at 45:55 (mobile phase); flow speed at 0.7 ml / min; wavelength at 254 nm; column temperature at 25 ° C. [Conclusion] The research preliminarily established HPLC conditions for separation of endogenous hormones in rhizome of Alhagi sparsifolia.