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目的：大鼠ZA73基因(GenBank accession number：AF011363)是本实验室从大鼠支气管上皮恶性转化细胞模型中采用mRNA差异显示技术克隆到的EST片段，与支气管上皮细胞恶性转化相关。根据此EST序列，克隆人全长基因。材料与方法：运用生物素标记探针筛选人cDNA文库、将筛选基因进行cDNA快速终末端扩增(Rapid amlification of cDNA ends)，直至获得全长序列。结果：HRNT-1 cDNA总长4256 bp，开放阅读框架2760 bp，5′非编码序列253bp，3′非编码序列1240 bp，已被GenBank收录于Nr数据库中(Accession number：AF223393)。结论：采用生物素标记cDNA文库筛选和cDNA快速终末端扩增相结合的技术是克隆全长cDNA快速而有效的方法，尤其适用于那些长度超过2kb的基因。
OBJECTIVE: The rat ZA73 gene (GenBank accession number: AF011363) is an EST fragment cloned from rat bronchial epithelial malignant transformed cell model by mRNA differential display technique, which is associated with the malignant transformation of bronchial epithelial cells. Based on this EST sequence, the human full-length gene was cloned. MATERIALS AND METHODS: Human cDNA library was screened with biotin-labeled probe, and the screening genes were subjected to Rapid amlification of cDNA ends until the full-length sequence was obtained. Results: The total length of HRNT-1 cDNA was 4256 bp with an open reading frame of 2760 bp, a 5 ’non-coding sequence of 253 bp and a 3’ non-coding sequence of 1240 bp. The cDNA sequence of HRNT-1 was registered in the Nr database by GenBank (Accession number: AF223393). Conclusion: The combination of biotinylated cDNA library screening and cDNA rapid terminal amplification is a rapid and effective method for cloning full-length cDNA, especially for those over 2kb in length.