论文部分内容阅读
目的:研究化合物E-2-(3’,5’-二甲氧基苯亚甲基)-环己酮(IV8)对人类宫颈癌(HeLa)、肝癌(Hep G2)、前列腺癌(Du145)和胃癌(SGC7901)细胞系的抗肿瘤活性。方法:采用MTT法评价IV8对上述4株肿瘤细胞株增殖的影响,并在光学显微镜下观察IV8对HepG2细胞形态学上的变化,以及采用AO/EB双荧光染色法评价IV8对HepG2细胞的凋亡作用。结果:IV8抑制HeLa,Hep G2,Du145和SGC7901细胞的IC50值分别为2.10,11.32,36.50和89.53μmol/L;光学显微镜下观察IV8处理的细胞出现明显增殖抑制和形态学改变;AO/EB双荧光染色IV8组可见凋亡细胞。结论:药理实验结果表明IV8具有较好的体外抗肿瘤的活性,值得进一步研究。
Objective: To study the effects of compound E-2- (3 ’, 5’-dimethoxybenzylidene) -cyclohexanone (IV8) on human cervical cancer (HeLa), liver cancer (Hep G2), prostate cancer (Du145) And gastric cancer (SGC7901) cell lines. Methods: The effects of IV8 on the proliferation of the four tumor cell lines were evaluated by MTT assay. The morphological changes of HepG2 cells were observed under optical microscope. The apoptosis of HepG2 cells was evaluated by AO / EB double staining Dead effect. Results: The IC50 of IV8 in HeLa, Hep G2, Du145 and SGC7901 cells were 2.10,11.32,36.50 and 89.53μmol / L, respectively. The proliferation and morphological changes of IV8-treated cells were observed under light microscope. Fluorescent staining of apoptotic cells in group IV8. Conclusion: Pharmacological results show that IV8 has good antitumor activity in vitro, worthy of further study.