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目的通过检测肿瘤坏死因子受体相关因子(TRAF-2)在骨肉瘤细胞表达的变化及三氧化二砷(As2O3)和血管内皮细胞生长因子(VEGF)对其表达的影响,探讨TRAF-2在骨肉瘤发病机制中的作用。方法人骨肉瘤Sa OS-2细胞经分别加入不同浓度的As2O3和(或)VEGF培养48 h后,采用实时荧光定量PCR法测定TRAF-2 mRNA的表达水平。结果 100 ng/ml浓度的VEGF能显著上调TRAF-2 mRNA的表达水平,差异有统计学意义(P<0.01),50 ng/ml以下浓度的VEGF对TRAF-2 mRNA的表达水平没有影响,差异无统计学意义(P>0.05);2μmol/L以上浓度的As2O3能显著下调TRAF-2 mRNA的表达水平,并能显著降低100 ng/ml浓度的VEGF对TRAF-2 mRNA表达水平的上调功能,差异有统计学意义(P<0.01);而1μmol/L浓度的As2O3对TRAF-2 mRNA的表达水平没有影响,并不影响100 ng/ml浓度的VEGF对TRAF-2 mRNA表达水平的诱导作用,差异无统计学意义(P>0.05)。结论 TRAF-2在骨肉瘤的发病机制中可能起重要作用,VEGF具有上调TRAF-2 mRNA表达水平的作用,而As2O3则起相反作用,两者具有一定的量-效关系。
Objective To detect the expression of TRAF-2 in osteosarcoma cells and the effect of arsenic trioxide (As2O3) and vascular endothelial growth factor (VEGF) on the expression of TRAF-2 in osteosarcoma Mechanism of the role. Methods Human osteosarcoma Sa OS-2 cells were cultured with different concentrations of As2O3 and / or VEGF for 48 h, respectively. The expression of TRAF-2 mRNA was detected by real-time fluorescence quantitative PCR. Results The level of TRAF-2 mRNA was significantly up-regulated by 100 ng / ml VEGF (P <0.01). The level of TRAF-2 mRNA was not affected by VEGF at the concentration of 50 ng / ml (P> 0.05). As2O3 at a concentration above 2μmol / L could significantly down-regulate the expression of TRAF-2 mRNA and down-regulate the TRAF-2 mRNA expression at a concentration of 100 ng / ml, (P <0.01). As2O3 at a concentration of 1μmol / L had no effect on the expression of TRAF-2 mRNA, but did not affect the induction of TRAF-2 mRNA at a concentration of 100ng / ml, The difference was not statistically significant (P> 0.05). Conclusions TRAF-2 may play an important role in the pathogenesis of osteosarcoma. VEGF has the effect of up-regulating the expression of TRAF-2 mRNA, while As2O3 has the opposite effect, both of which have a certain dose-response relationship.