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背景:原代培养的破骨细胞数量少,而诱导培养产生的破骨样细胞数量多,能够满足一些研究骨代谢实验的要求。但是两种细胞在特异酶及噬骨能力方面是否具有相同的效果,到目前为止,还没有详尽的数据来支持。目的:比较大鼠原代分离的破骨细胞及诱导形成的破骨样细胞噬骨能力上的差异。方法:取24h内新生Wistar大鼠四肢长骨,酶消化法分离培养破骨细胞,将破骨细胞培养过程中消化下来的骨髓单核细胞加入1,25(OH)2D3诱导生成破骨样细胞。苏木精-伊红染色、抗酒石酸酸性磷酸酶(TRAP)染色鉴定。用甲苯胺蓝染色共培养骨片比较两组破骨细胞噬骨能力。结果与结论:诱导第9天,破骨样细胞数目为破骨细胞数目的11倍,其形态与原代消化获得的细胞形态相同。抗酒石酸酸性磷酸酶染色呈阳性。甲苯胺蓝染色显示两组破骨细胞在骨片上培养时产生骨陷凹面积及深度差异无显著性意义。结果显示破骨样细胞的形态、特异酶及噬骨能力与破骨细胞无差异。
Background: The number of osteoclasts cultured in primary culture is small, while the number of osteoclast-like cells produced by induction culture is large, which can meet the requirements of some experiments on bone metabolism. However, whether the two cells have the same effect on the specific enzyme and the ability to bite the bone has so far not been fully supported. OBJECTIVE: To compare the ability of primary osteoclasts isolated from rats and induced osteoclastic osteoclasts to differentiate into macrophages. Methods: Osteoclasts were isolated and cultured from freshly born Wistar rats for 24 hours. The osteoblasts were induced by addition of 1,25 (OH) 2D3 to the bone marrow mononuclear cells. Hematoxylin-eosin staining and tartrate-resistant acid phosphatase (TRAP) staining. Co-cultured bone slices were stained with toluidine blue to compare the osteoclastic bone-marrow capacity of the two groups. RESULTS AND CONCLUSION: On the 9th day of induction, the number of osteoclast-like cells was 11 times of the number of osteoclasts. The morphology of the osteoclast-like cells was the same as that obtained from the primary digestion. Tartrate-resistant acid phosphatase staining was positive. Toluidine blue staining showed that there was no significant difference between the two groups in the area and depth of osteoclasts produced when the osteoclasts were cultured on the bone slices. The results showed that the shape of osteoclast-like cells, specific enzyme and bone-grafting ability and no difference between osteoclasts.