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目的探讨人巨细胞病毒(human cytomegalovirus,HCMV)感染在结直肠癌发生、发展过程中可能的作用机制。方法收集50例结直肠癌患者的病理肿瘤组织标本,同时收集距离肿瘤组织手术切缘10cm以上的自身瘤旁正常肠粘膜组织为对照,应用RT-PCR技术检测组织中HCMV的感染情况,免疫组化技术检测凋亡相关基因Bcl-2的表达水平。结果肿瘤组及瘤旁组HCMV IE2阳性率分别为40.00%(20/50)和6.00%(3/50),差异有统计学意义(P<0.05);Bcl-2阳性率分别为36.00%(18/50)和8.00%(4/50),差异有统计学意义(P<0.05)。50例HCMV阳性肿瘤组织Bcl-2阳性率高于HCMV阴性肿瘤组织,肿瘤组织Bcl-2表达水平与HCMV感染呈线性相关(χ2=5.834,P<0.05)。结论 HCMV感染是结直肠癌发生、发展的相关因素之一,其作用机制与影响Bcl-2的表达有关。
Objective To investigate the possible mechanism of human cytomegalovirus (HCMV) infection in the development and progression of colorectal cancer. Methods The pathological tumor samples of 50 colorectal cancer patients were collected. At the same time, the normal intestinal mucosa tissue adjacent to the surgical margins more than 10 cm away from the tumor tissue was collected as a control. RT-PCR was used to detect the HCMV infection in the tissues. The expression level of apoptosis-related gene Bcl-2 was detected by the technique. Results The positive rates of HCMV IE2 were 40.00% (20/50) and 6.00% (3/50) in the tumor and paratumor groups, respectively. The difference was statistically significant (P<0.05); the positive rate of Bcl-2 was 36.00% ( 18/50) and 8.00% (4/50), the difference was statistically significant (P<0.05). The positive rate of Bcl-2 in 50 cases of HCMV positive tumor tissue was higher than that of HCMV negative tumor tissue. The expression of Bcl-2 in tumor tissue was linearly correlated with HCMV infection (χ2=5.834, P<0.05). Conclusion HCMV infection is one of the related factors in the occurrence and development of colorectal cancer. Its mechanism of action is related to the expression of Bcl-2.