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目的 研究 β 环糊精 14硫酸酯 (β CD 14S)抑制肿瘤新生血管的作用机制。 方法 采用3 H 胸腺嘧啶核苷 (3 H TdR)掺入法 ,检测 β CD 14S单独及与氢化可的松 (HC)联合应用 (以各种药物加药浓度不同分别分为 5个组 )对血管内皮细胞、正常肝细胞系 (L0 2 )细胞 ,人肝癌 772 1细胞和肝癌Waker 2 5 6细胞增殖的影响。结果 各种不同浓度的 β CD 14S、HC以及联合用药对内皮细胞生长率有明显的抑制 ;与L0 2 ,772 1,Waker 2 5 6细胞相比较 ,差异均有显著性 (P <0 .0 0 1)。联合用药较单独用药对内皮细胞生长率的抑制更加明显 (P <0 .0 0 1)。结论 β CD 14S具有抑制新生血管形成和药物靶向载体的双重功能。
Objective To study the mechanism of β CD 14S inhibiting tumor neovascularization. Methods 3 H TdR incorporation method was used to detect β CD 14S alone and in combination with hydrocortisone (HC), which was divided into 5 groups according to different drug concentration Vascular endothelial cells, normal liver cell line (L0 2) cells, human hepatocellular carcinoma 772 1 cells and hepatocellular carcinoma Waker 2 5 6 cells. Results Different concentrations of β CD 14S, HC and combined treatment significantly inhibited the growth of endothelial cells. Compared with L0 2, 772 1 and Waker 2 5 6 cells, the differences were significant (P <0. 0 0 1). The combination of drugs inhibited the growth of endothelial cells more significantly than alone (P <0.01). Conclusion β CD 14S has the dual function of inhibiting neovascularization and drug targeting vector.