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从广东省12个县、市采集了20个桑青枯菌株进行血清学反应、生化反应、寄主范围、烟叶浸润反应、酪氨酸酶活性、致病性、溶源性和菌落特性等试验,并按前人的研究方法划分为相应的血清型、生化型、小种、致病型、溶源型和菌落型等,但这些分型相互间无一定的联系。根据血清学反应的结果,我们把20个桑青枯菌株和花生、番茄、木麻黄和马铃薯等青枯菌株总共划分为8个血清型,桑青枯菌的20个菌株分属于4个血清型。这些桑青枯菌株与花生、番茄和木麻黄等青枯菌株的血清学关系比与马铃薯青枯菌株的关系较为密切。据此我们认为青枯菌种下的分类应以血清型作为主要的分类依据。在血清型下,依次为生化型、小种和致病型等。
Twenty isolates of R. solani were collected from 12 counties and cities in Guangdong Province for serological, biochemical, host range, tobacco leaf infiltration, tyrosinase activity, pathogenicity, lysogenic and colony characteristics, According to the previous research methods, they were divided into corresponding serotype, biochemical, races, pathogenic, lysogenic and colony types, but these types were not related to each other. According to the results of serological reaction, we divided 20 R. solanacearum strains and 8 strains of R. solanacearum such as peanut, tomato, casuarina and potato into 20 serotypes. 20 strains of R. solani belonged to 4 serotypes . The serological relationships between these Pseudomonas solanacearum strains and the strains of R. solanacearum, such as peanut, tomato and Casuarina, were more closely related to the strains of R. solanacearum. Therefore, we believe that the classification of R. solanacearum should be serotype as the main classification basis. In the serotypes, followed by biochemical, race and pathogenic type.