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目的 :用 HPL C法测定心元胶囊中大黄素与丹参酮 A 的含量。方法 :样品的甲醇提取物经酸水解用乙醚提取后 ,以 ODS柱分析 ,大黄素的测定 ,流动相为甲醇 -水 -磷酸 (80∶ 2 0∶ 0 .0 5 ) ,检测波长为 2 86 nm;丹参酮 A的测定 ,流动相为甲醇 -水 (80∶ 2 0 ) ,检测波长为 2 6 8nm。结果 :大黄素的线性范围为 0 .0 2 13～ 0 .2 13μg(r=0 .9999) ,样品加样回收率为 99.85 % ,RSD为 1.95 % (n=6 ) ;丹参酮 A 的线性范围为 0 .0 12～ 0 .0 5 9μg(r=0 .9993) ,样品加样回收率为 97.3% ,RSD为 1.3% (n=6 )。结论 :该法可供该制剂含量测定
Objective: To determine the content of emodin and tanshinone A in Xin yuan capsule by HPL C method. Methods: The methanol extract of the sample was extracted by ether with acid hydrolysis and analyzed by ODS column. The emodin was determined by the mobile phase of methanol-water-phosphoric acid (80:20:50) and the detection wavelength was 2 86 nm; The determination of tanshinone A, the mobile phase is methanol-water (80:20), detection wavelength is 2680nm. Results: The linear range of emodin was 0.022-13.0.2 13μg (r = 0.9999). The sample recovery was 99.85% with RSD 1.95% (n = 6). The linear range of tanshinone A Was 0.12 ~ 0.059 μg (r = 0.9993). The sample recovery was 97.3% and the RSD was 1.3% (n = 6). Conclusion: The method for the determination of the content of the preparation