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目的采集手术切除未经培养的骨肉瘤标本,研究肿瘤中成骨细胞的分化及其与临床因素的关系。方法观察骨肉瘤中成骨细胞相关基因表达的频率、特异性,以及骨肉瘤成骨细胞表型与生物学行为的关系。应用RT-PCR方法研究48例骨肉瘤标本的碱性磷酸酶(ALP)、骨钙素(OC)和骨桥蛋白(ON)的mRNA转录。磷酸甘油酸激酶(PGK)被用于内参照。结果在大多数骨肉瘤标本中可以发现ALP或OC的mRNA转录(分别为93.75%,79.17%)。在所有的肉瘤检测中均可发现ON的mRNA转录。在骨肉瘤的成骨细胞亚型中可见到ALP和OC的高水平表达。肺转移瘤OC的mRNA表达弱于原发灶。肿瘤细胞有高水平OC表达的病例组有较高的生存率(P=0.045)。结论OC是目前最好的成骨细胞特异性标记物,将来可能用于检测骨肉瘤患者外周血中循环的肿瘤细胞。
Objective To investigate the osteosarcoma differentiation and its relationship with clinical factors in surgical excision of uncultured osteosarcoma specimens. Methods The frequency, specificity of osteoblast-related gene expression in osteosarcoma and the relationship between osteoblast phenotype and biological behavior in osteosarcoma were observed. The mRNA transcription of alkaline phosphatase (ALP), osteocalcin (OC) and osteopontin (ON) in 48 osteosarcoma specimens was studied by RT-PCR. Phosphoglycerate kinase (PGK) was used for the internal reference. Results mRNA transcripts of ALP or OC were found in most osteosarcoma specimens (93.75%, 79.17%, respectively). ON mRNA transcription can be found in all sarcomas. High levels of ALP and OC expression are seen in the osteoblastic subtype of osteosarcoma. Lung metastases OC mRNA expression was weaker than the primary tumor. The tumor group had a higher survival rate (p = 0.045) in patients with high OC levels. Conclusion OC is the best osteoblast-specific marker in the future and may be used to detect circulating tumor cells in the peripheral blood of patients with osteosarcoma in the future.