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在单克隆抗体(McAb)制备工作中,可用毫微克(ng)的抗原量对小鼠脾细胞进行体外免疫,但此法需要复杂的培养条件以保持细胞的活性。本文报告了应用BALB/c小鼠淋巴结初次免疫技术,只需100ng牛血清白蛋白(BSA)的抗原量就能产生多克隆抗体,用少于20μg的抗原量(从用疱疹病毒转化的仓鼠肾细胞提取的35KD多肽)即能产生McAb。用苯妥英钠麻醉小鼠,打开腹腔,用解剖显微镜找到腹腔淋巴结,并用显微操作器上的细长毛细管向淋巴结中注入1μl弗氏完全佐剂抗原(牛血清白蛋白抗原溶液与佐剂
In monoclonal antibody preparation, mouse splenocytes can be immunized in vitro with antigenic amounts of ng, but this method requires complex culture conditions to maintain cellular activity. This article reports the use of primary immunization of lymph nodes in BALB / c mice to generate polyclonal antibodies against as little as 100 ng of BSA antigen and less than 20 μg of antigen (from hamster kidney transformed with herpesviruses Cell extract 35KD polypeptide) that is able to produce McAb. Mice were anesthetized with phenytoin, the abdominal cavity was opened, the abdominal lymph nodes were found with a dissecting microscope, and 1 μl Freund’s complete adjuvant antigen (bovine serum albumin antigen solution and adjuvant) was injected into the lymph nodes using slender capillaries on a micromanipulator