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作者介绍了一种供公共卫生和临床实验室使用的、用ELISA检测百日咳毒素(PT)抗体的新的实用方法。该法不需要高度纯化的PT抗原,且所用试剂易购买。该法要点如下:(1)使百日咳杆菌在培养液中生长,得到富含PT的上清液。培养液A液每升含10g酪蛋白氨基酸,1g heptakis(2,6-O-二甲基)β-环糊精,2.5g甘油磷酸钠10.7g L-谷氨酸钠,0.24g L-脯氨酸,2.5g NaCl,0.5g KH_2PO_4,0.2g KCl,0.(?)g MgCl_2(6H_2O)和0.02g CaCl_2。B液含
The authors present a new practical method for the detection of pertussis toxin (PT) antibodies by ELISA for use in public health and clinical laboratories. This method does not require highly purified PT antigen, and reagents are easy to buy. The main points of this method are as follows: (1) Bordetella pertussis is grown in a culture solution to obtain a PT-rich supernatant. The culture liquid A contains 10 g of casein amino acids per liter, 1 g of heptakis (2,6-O-dimethyl) β-cyclodextrin, 2.5 g of sodium glycerophosphate, 10.7 g of sodium L-glutamate, 0.24 g of L- 2.5 g NaCl, 0.5 g KH 2 PO 4, 0.2 g KCl, 0. g MgCl 2 (6H 2 O) and 0.02 g CaCl 2. B liquid containing