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目的 :探讨白细胞在新生大白鼠窒息后肾损伤中的作用及机制。方法 :将 2 0只 7～ 10日龄 Wistar大白鼠随机分为窒息组和对照组 ,每组 10只 ,制成常压窒息模型。在窒息 30分钟复氧 12 0分钟时 ,取左肾称重求出左肾系数 (L RC) ,并分别采用硫代巴比妥酸法、酶联免疫吸附法 (EL ISA)检测肾组织过氧化脂质 (L PO)、肿瘤坏死因子α(TNFα)和白介素 8(IL 8)的含量 ,同时在光镜下对肾小管损伤程度进行评分 ,并计数肾微血管床的白细胞滞留数。结果 :窒息后复氧 12 0分钟时 ,白细胞滞留数、左肾系数、肾小管评分及组织中 L PO、TNFα、IL 8的含量均较对照组显著升高 (P均 <0 .0 5 ) ,且白细胞滞留数与左肾系数、肾小管评分、L PO、TNFα和 IL 8含量之间呈显著正相关关系 (r分别为 0 .49、0 .5 5、0 .5 1、0 .48、0 .70 ,P均 <0 .0 5 )。结论 :窒息后肾组织炎性细胞因子的产生和白细胞滞留相互作用是造成窒息后肾损伤的重要环节 ,对临床上采用抗炎或抗白细胞措施防治窒息后肾损伤有重要价值。
Objective: To investigate the role and mechanism of leucocyte in renal injury induced by asphyxia in neonatal rats. Methods: Twenty male Wistar rats aged 7-10 days were randomly divided into asphyxia group and control group, with 10 rats in each group. In the asphyxial 30 minutes reoxygenation 120 minutes, the left kidney was weighed to determine the left renal coefficient (L RC), respectively, and the use of thiobarbituric acid method, enzyme-linked immunosorbent assay (EL ISA) (L PO), tumor necrosis factor α (TNFα) and interleukin 8 (IL 8) were measured. The extent of renal tubular injury was scored under light microscope, and the number of leukocytes in renal microvascular bed was counted. Results: The levels of leukocyte retention, left renal index, tubule score, and levels of L PO, TNFα and IL-8 in the reoxygenation group after 12-hour reoxygenation were significantly higher than those in the control group (all P <0.05) , And there was a significant positive correlation between leukocyte retention and left kidney index, tubule score, L PO, TNFα and IL 8 (r = 0.49,0.55,0.5,01.0.48 , 0.70, all P <0.05). Conclusion: Asphyxial renal tissue production of inflammatory cytokines and leucocyte retention interaction is an important part of renal injury caused by asphyxia. It is of great value in the clinical use of anti-inflammatory or anti-leukocyte measures to prevent and treat post-asphyxial renal injury.