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目的观察甲氰菊酯(Fenpropathrin,Fen)单独腹腔注射及与多巴胺(Dopamine,DA)立体定向注射联合使用对C57BL小鼠黑质纹状体多巴胺能神经通路的影响。方法分别采用Fen腹腔注射、DA立体定向注射至纹状体及DA预处理联合Fen注射建立C57BL小鼠模型,观察小鼠行为学变化,HPLC检测脑组织内Fen的含量;激光共聚焦显微镜观察小鼠的黑质多巴胺能神经元及纹状体的TH染色。结果 Fen及DA分别单独使用及DA预处理联合Fen注射均造成小鼠自主活动能力减少,HPLC检测显示小鼠脑组织中Fen的浓度与腹腔Fen给药浓度成正比,TH染色发现Fen连续使用7 d和DA立体定向注射后第7 d的小鼠黑质均出现神经元中TH表达不均一,并且纹状体出现斑片状的TH染色丢失,其中DA预处理后使用Fen注射7d组改变最明显。结论 Fen能够透过小鼠血脑屏障,可以直接作用于小鼠黑质纹状体多巴胺能系统,并且可以明显增强DA对黑质纹状体的损伤作用。
Objective To observe the effects of Fenpropathrin (Fen) alone and intraperitoneal injection with dopamine (Dopamine, DA) on dopaminergic neuronal pathways in the substantia nigra and striatum of C57BL mice. Methods C57BL mouse model was established by intraperitoneal injection of Fen, DA stereotactic injection into the striatum and DA pretreatment combined with Fen injection. The behavioral changes of the mice were observed. The content of Fen in the brain tissue was detected by HPLC. The laser confocal microscopy TH-staining of rat substantia nigra dopaminergic neurons and striatum. Results Fen and DA alone and DA pretreatment combined with Fen injection caused decreased ability of autonomic activity in mice. HPLC analysis showed that the concentration of Fen in the brain tissue of mice was proportional to the concentration of Fen administered intraperitoneally. The TH staining showed that Fen was used continuously for 7 At 7 days after stereotactic injection of d and DA, there was heterogeneity of TH expression in the substantia nigra of neurons, and plaque-like TH staining of the striatum was lost. After DA pretreatment, the rats injected with Fen for 7 days changed the most obvious. Conclusion Fen can penetrate the blood-brain barrier in mice and can act directly on the nigrostriatal dopaminergic system in mice, and can significantly enhance the damaging effect of DA on nigrostriatum.