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目的采用UPLC法测定手参中6种天麻素类成分的含量。方法色谱柱为Waters BEH C18柱(50 mm×2.1 mm,1.7μm),流动相为乙腈-0.1%磷酸,梯度洗脱,流速0.4 m L·min-1,检测波长222 nm,柱温35℃。结果天麻素、天麻苷元、dactylorhin B、loroglossin、dactylorhin A、militarine的线性范围分别为0.99~21.80、0.57~12.59、1.90~41.92、1.37~30.10、1.99~43.72、3.00~16.50 ng,r分别为0.9998、0.9998、0.9997、0.9997、0.9999、0.9998;平均回收率分别为99.3%、96.8%、97.7%、96.1%、97.3%、95.8%,RSD分别为1.20%、2.72%、2.86%、1.67%、2.61%、2.40%(n=6)。结论所用方法准确、简便、重复性好,可快速测定手参中天麻素类成分的含量。
Objective To determine the content of six kinds of gastrodin in ginseng by UPLC. METHODS The column was a Waters BEH C18 column (50 mm × 2.1 mm, 1.7 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution at a flow rate of 0.4 mL · min-1. The detection wavelength was set at 222 nm and the column temperature was 35 ℃ . Results The linear ranges of dactylorhin B, loroglossin, dactylorhin A and militarine were 0.99 ~ 21.80,0.57 ~ 12.59,1.90 ~ 41.92,1.37 ~ 30.10,1.99 ~ 43.72,3.00 ~ 16.50 ng, r The average recoveries were 99.3%, 96.8%, 97.7%, 96.1%, 97.3% and 95.8%, respectively, and the RSDs were 1.20%, 2.72%, 2.86% and 1.67% 2.61%, 2.40% (n = 6). Conclusion The method used is accurate, simple and reproducible, and the content of gastrodin in ginseng can be quickly determined.