【摘 要】
：
Virus-induced gene silencing (VIGS) is a natural defense mechanism of plants, which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome. At present, the VIGS system mediated by Tobacco rattle virus (TR
【机 构】
：
College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 35002,China;College of Hor
论文部分内容阅读
Virus-induced gene silencing (VIGS) is a natural defense mechanism of plants, which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully established in many plant species. However, the VIGS system in Chinese narcissus had not been reported yet. In this paper, the first use of the TRV-VIGS system in Chinese narcissus is described in detail. By injecting pTRV2-GFP into narcissus leaves, we confirmed that TRV could infect narcissus and move in narcissus plants. After the inoculation of pTRV2-NtPDS by two methods, phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully silenced. Besides, pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3;the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis, which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of NtFLS. These results indicate that the TRV-VIGS system has been successfully established in Chinese narcissus and successfully applied to the functional study of NtMYB3.
其他文献
目的 系统评价针灸对胃癌患者化疗期间不良反应的临床疗效.方法 通过计算机检索中文期刊全文数据库(CNKI)、中国生物医学文献数据库(CBM)、维普期刊数据库(VIP)、万方医学网(WANFANG)和PubMed、MEDLINE、Web of Science数据库建库至2021年1月的针灸治疗胃癌患者化疗期间不良反应的临床随机对照试验研究文献,运用RevMan5.3软件进行Meta分析.结果 筛选出文献350篇,根据文献纳入排除标准,排除343篇,最终纳入7篇临床研究,共计484例患者,其中治疗组242例,
程序性死亡受体1(programmed cell death protein 1,PD-1)抑制剂能够阻断肿瘤细胞与T细胞之间的免疫抑制信号,激活人体的抗肿瘤活性,目前有临床研究将其应用于晚期肺癌患者中.肺癌的发病特点以“痰、瘀、毒、虚”为主,根据中医学理论,结合临床经验,治法以“益气除痰”为主,加以扶正固本.本报道应用益气除痰中药配合PD-1单药治疗PD-L1表达阴性的晚期肺腺癌1例,总生存期现已达4年.
本文报道介绍一例临床接诊的结肠腺癌术后化疗后继发Lambert-Eaton肌无力综合征的罕见病例,经由中药处方五苓散合补中益气汤加减治疗后患者肌无力症状得以控制,摆脱轮椅,生存质量提高.诊疗过程中主要运用的《黄帝内经》“治痿独取阳明”相关理论,通过辨证论治,取掉较好疗效,本文就疾病机理、中医辨治思路进行探讨,以供临床参考.
靶向药物在恶性肿瘤诊疗领域开启了精准治疗时代,因其以攻击肿瘤细胞为主,对人体未突变细胞影响较小,成为有阳性靶点肿瘤患者的首选诊疗手段.但相关皮肤毒性反应在临床中常会影响药物使用的连续性,降低抗肿瘤效果,极大影响患者的生存及预后.西医有关靶向药物所致皮肤毒性反应有诸多理论,但相关病理机制仍处于假说阶段.其临床治疗的有效方法多涉及药物剂量和给药周期的调整,抗肿瘤效果往往大打折扣.中医药治疗靶向药物相关手足皮肤反应,具有较好的疗效.本文就靶向药物相关手足皮肤反应的中医理论及治疗进行综述,以供临床参考.
癌性厌食恶病质综合征是与癌症本身及抗癌治疗有关的复杂性代谢综合征,是晚期恶性肿瘤的严重并发症,是导致患者死亡的重要原因,厌食、机体代谢异常、细胞因子变化等促使了癌性厌食恶病质综合征的发生.本文对癌性厌食恶病质综合征的中西医治疗,进行综述分析,以期为临床提供参考.
目的 运用网络药理学方法探讨消岩汤中夏枯草与白花蛇舌草治疗非小细胞肺癌的功效物质基础和作用机制.方法 运用中药系统药理学分析平台(traditional Chinese medicine systems phannacology,TCMSP)检索消岩汤中夏枯草与白花蛇舌草两味中药的活性化合物与作用靶点,通过Online Mendelian Inheritance in Man(OMIM)、Genecards、PharmGkb以及治疗靶点数据库(Therapeutic Target Database,TTD
近年来,互联网移动医疗的发展呈现出快速上升的趋势,其在中医健康管理方面的应用价值也日益受到重视.中医药对于肺癌的防治具有显著的特色和优势,在我国肺癌的诊疗领域发挥了重要作用.我们基于互联网移动医疗,引入整体观念、辨证论治、体质、治未病与养生康复等中医核心思想,研制适用于肺癌的中医健康管理应用程序“肺i中医”和肺癌中医健康管理网络数据系统,给广大肺癌患者介绍中医药防治肺癌的理论知识,提供专业的肺癌证候辨识和体质识别,给予个体化的方药及针灸治疗方案,优选药膳和行为干预,康复锻炼的说明与指导等,并推出在线诊疗服
Mesocarp color is an important agronomic trait of Cucurbita maxima and is determined mainly by the contents and compositions of the carotenoids. The two inbred lines with significant differences in fruit flesh color were used in the study, the orange\'3
Plant viruses are mainly transmitted by insect vectors in the non-persistent, semi-persistent, or persistent modes. In the non-persistent mode, plant viruses are retained in the stylets of their insect vectors. In the semi-persistent mode, plant viruses a
The various monochromatic Light Emitting Diode (LED) lights are widely used in growth facility for cultivating various plants, particularly horticultural crops because of their higher luminous efficiency, lower radiation and power consumption than the tra