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[目的]获得可在低温环境下高效降解粪污水的芽孢杆菌。[方法]从大型养殖场及活性污泥、下水道等低温环境下采集样品,分离并筛选耐冷菌株,通过扫描电镜观察获得菌株的形态特征,分析其16S rRNA基因测序,构建系统发育树,初步确定菌株的菌属;对其进行鉴定后将其添加到污水生物反应器中对猪场粪污水进行净化,分析粪污水中化学需氧量(COD)、生物需氧量(BOD)及溶解氧(DO)变化情况。[结果]这4株菌均为蜡样芽孢杆菌;在粪污水里添加这4株菌的复合菌液后,粪污水的COD值从5 585.40 mg/L降至1 093.97 mg/L,去除率达80.40%;BOD值从680.00 mg/L降至600.00 mg/L,去除率为11.76%;DO值大幅度上升,增加率为75.00%。[结论]复合菌在粪污水处理方面表现出了较高的降解能力,可为污染物生物修复提供优良的菌种资源,具有广阔的应用前景。
[Objective] The research aimed to obtain Bacillus that can efficiently decontaminate waste water in low temperature environment. [Method] The samples were collected from large-scale farms, activated sludge, sewers and other low-temperature environments. The cold-resistant strains were isolated and screened. The morphological characteristics of the strains were obtained by scanning electron microscopy. The 16S rRNA gene sequences were analyzed and phylogenetic trees were constructed. Staphylococcus aureus and Staphylococcus aureus were identified. After being identified, they were added to the wastewater bioreactor to purify the excrement and sewage from pig farms. The COD, BOD and DO (dioxygenase) DO) changes. [Result] The four strains were Bacillus cereus. After adding these four strains of compound bacteria in sewage water, the COD value of waste water dropped from 5858.40 mg / L to 1093.97 mg / L, and the removal rate Reaching 80.40%. The BOD value decreased from 680.00 mg / L to 600.00 mg / L, the removal rate was 11.76%. The DO value increased greatly with the increase rate of 75.00%. [Conclusion] The composite bacteria showed high degradation ability in the treatment of excrement water, which could provide excellent bacteria resources for the bioremediation of pollutants and had broad application prospect.