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目的探讨丙戊酸钠(SVPA)和阿霉素(ADM)对人骨髓增生异常综合征(MDS)MUTZ-1细胞生长抑制的协同作用。方法 MUTZ-1细胞分为对照组(A组)、SVPA组(B组)、ADM组(C组)、SVPA+ADM组(D组)和SVPA预处理12 h+ADM组(E组)。四甲基偶氮唑蓝比色(MTT)法观察药物对细胞生长的影响;RT-PCR检测TOPOIIαmRNA表达水平。结果 B、C组对细胞生长的抑制作用均明显强于A组(P<0.05);与C组相比,D组和E组细胞生长的抑制率明显增加(P<0.05);但D组和E组间差异没有统计学意义(P>0.05)。与A组相比,B、C、D组明显增加TOPOIIαmRNA表达(P<0.05);D组较C组更能有效诱导TOPOIIα表达上调(P<0.05)。结论 SVPA可增强ADM对人MDS MUTZ-1细胞生长的抑制作用;此协同作用可能与TOPOIIαmRNA表达的上调有关。
Objective To investigate the synergistic effect of sodium valproate (SVPA) and doxorubicin (ADM) on the growth inhibition of human myelodysplastic syndrome (MDS) MUTZ-1 cells. Methods MUTZ-1 cells were divided into control group (group A), SVPA group (group B), ADM group (group C), SVPA + ADM group (group D) and SVPA pretreatment group (group E) for 12 h. The effects of drugs on cell growth were observed by MTT method. The expression of TOPOIIα mRNA was detected by RT-PCR. Results The inhibition of cell growth was significantly stronger in group B and C than that in group A (P <0.05). Compared with group C, the inhibition rates of cell growth in group D and group E were significantly increased (P <0.05) There was no significant difference between group E and group E (P> 0.05). Compared with group A, the expression of TOPOIIαmRNA in group B, C and D increased significantly (P <0.05); group D increased the expression of TOPOIIα more effectively than group C (P <0.05). Conclusions SVPA can enhance the inhibitory effect of ADM on human MDS MUTZ-1 cells. This synergistic effect may be related to the up-regulation of TOPOIIα mRNA expression.