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建立了超高效液相色谱法测定大鼠血浆中的酒石酸美托洛尔,并考察盐酸沙格雷酯对酒石酸美托洛尔在大鼠体内药动学的影响。选用拉莫三嗪为内标,Acquity UPLC~174;BEH C_(18)色谱柱,以甲醇∶0.01 mol/L磷酸二氢钾缓冲液(加磷酸调至p H 3.6)(31∶69)为流动相,检测波长225 nm。40只Wistar大鼠随机分为2组,每组20只,对照组灌胃给予酒石酸美托洛尔27 mg/kg,试验组灌胃给予盐酸沙格雷酯10 mg/kg和酒石酸美托洛尔27 mg/kg。用DAS 2.1.1软件拟合药动学参数,并以SPSS 13.1软件比较2组的药动学参数。结果表明,试验组的AUC_(0→5 h)、AUC_(0→∞)、t_(1/2)、t_(max)、V、CL和c_(max)与对照组相比无显著性差异(P>0.05)。合用盐酸沙格雷酯前后酒石酸美托洛尔在大鼠体内的药动学无显著性变化。
To establish the ultra performance liquid chromatographic method for the determination of metoprolol tartrate in rat plasma and to study the effect of sarpogrelate hydrochloride on the pharmacokinetics of metoprolol tartrate in rats. Using lamotrigine as internal standard, the cells were separated on a Acquity UPLC ~ 174; BEH C_ (18) column with methanol: 0.01 mol / L potassium dihydrogen phosphate buffer (adjusted to pH 3.6 with phosphoric acid) (31:69) As mobile phase, detection wavelength of 225 nm. Forty Wistar rats were randomly divided into two groups (20 rats in each group). The control group was intragastrically given metoprolol tartrate 27 mg / kg. The test group was given sogrel hydrochloride 10 mg / kg and metoprolol tartrate 27 mg / kg. Pharmacokinetic parameters were fitted using DAS 2.1.1 software and pharmacokinetic parameters were compared between the two groups using SPSS 13.1 software. The results showed that the AUC_ (0 → 5 h), AUC_ (0 → ∞), t_ (1/2), t_ (max), V, CL and c_max of the experimental group showed no significant difference compared with the control group (P> 0.05). There was no significant change in the pharmacokinetics of metoprolol tartrate before and after administration of sarpogrelate hydrochloride in rats.