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叶黄素循环在逆境胁迫中对植物光系统起着重要的保护作用。紫黄质脱环氧化酶(VDE)和玉米黄质环氧化酶(ZEP)是调控叶黄素循环的关键酶。本研究采用RT-PCR和RACE技术率先从草莓中克隆获得VDE和ZEP基因。VDE基因c DNA全长1 842 bp,ORF为1 467 bp,编码489个氨基酸;ZEP基因c DNA全长2 325 bp,ORF为1 980 bp,编码660个氨基酸。生物信息学分析表明草莓VDE与ZEP基因所编码的氨基酸序列与蔷薇科的苹果、桃和梅等植物的亲缘关系较近。荧光定量分析发现VDE和ZEP基因在草莓根、茎、叶、花、果实中皆有表达,说明二者均为组成型表达基因,并且叶片中基因的表达量最高而根中最低,推测与其在草莓叶黄素循环调控的作用有关。
Lutein cycle plays an important protective role in plant photosystem under stress. Violaxanthogen deoxidized (VDE) and zeaxanthin cyclooxygenase (ZEP) are key enzymes that regulate the lutein cycle. In this study, VDE and ZEP genes were first cloned from strawberry using RT-PCR and RACE techniques. The cDNA of VDE gene was 1 842 bp in length with an ORF of 1 467 bp encoding a protein of 489 amino acids. The cDNA of ZEP gene was 2 325 bp in length with an ORF of 1 980 bp encoding 660 amino acids. Bioinformatics analysis showed that the amino acid sequences encoded by strawberry VDE and ZEP genes are closely related to Rosmarinoses such as apple, peach and plum. Fluorescence quantitative analysis showed that VDE and ZEP genes were expressed in roots, stems, leaves, flowers and fruits of strawberry, indicating that both of them were constitutively expressed genes and the genes in leaves had the highest expression and the lowest in roots, Strawberry lutein cycle regulation of the role.