论文部分内容阅读
目的 :评估卵巢肿瘤细胞凋亡与增殖状况及两者的关系。方法 :运用流式细胞术检测良交恶三组卵巢肿瘤中bcl-2、p16、Rb,Cyclin D1蛋白及 TGF-β1 因子的表达和 DNA含量 (恶性组 3 3例、交界组 12例、良性组 5例 )。各指标间的相关性通过单因素直线相关分析和多因素直线相关分析来评估。结果 :bcl-2和 AP%间 ,TGF-β1 和 PI间俱呈负相关 ( r=-0 .2 8,P<0 .0 5 ,r=-0 .2 5 ,P<0 .0 5 )。多因素相关分析发现 p16的 F I值和 PI值呈负相关 ( Yi=-2 0 .3 2 ,P=0 .0 81)。恶性组和交界组卵巢肿瘤的 AP%值与良性肿瘤比较发生了相对减少。多因素分析证实 A P%值与 DI值呈负相关 ( Yi=-9.96,P=0 .0 3 7)。恶性组的细胞累积率高于良性组 ( P<0 .0 1)和交界组 ( P<0 .0 5 )。bcl-2和 p16呈负相关 ( r=-0 .2 6,P<0 .0 5 )。结论 :bc L -2能够抑制凋亡 ,它的增加有助于细胞恶性转变。 TGF-β1 能够抑制增殖 ,细胞对 TGF -β1 反应性的降低有助于恶变。 p16、 Rb的缺失和 Cyciln D1的增加有助于卵巢癌的发生和发展。恶性肿瘤中 AP%值的相对下降对于肿瘤恶变具有重要意义。 bcl-2和 p16间的负相关提示凋亡和增殖间具有相互作用。正由于凋亡相关蛋白和细胞周期调节因子间的相互作用 ,使恶性肿瘤保持较高的细胞累积率。
Objective: To evaluate the relationship between ovarian tumor cell apoptosis and proliferation and their relationship. Methods: Flow cytometry was used to detect the expression of bcl-2, p16, Rb, Cyclin D1 and TGF-β1 and the DNA content in benign and malignant ovarian tumors (33 cases in malignant group, 12 cases in benign group, 5 cases). Correlation between the indicators by single factor linear correlation analysis and multivariate linear correlation analysis to assess. Results: There was a negative correlation between TGF-β1 and PI between bcl-2 and AP% (r = -0.28, P <0.05, r = -0.25, P <0.05 ). Multivariate correlation analysis showed that the PI value of p16 was negatively correlated with PI (Yi = -2.0.32, P = 0.081). Ovarian tumors in the malignant and borderline groups showed a relative decrease in AP% compared with benign tumors. Multivariate analysis confirmed that A P% was negatively correlated with DI (Yi = -9.96, P = .037). The cell accumulation rate in malignant group was higher than that in benign group (P <0.01) and junctional group (P <0.05). There was a negative correlation between bcl-2 and p16 (r = -0.26, P <0.05). Conclusion: bc L -2 can inhibit apoptosis, and its increase contributes to the malignant transformation of cells. TGF-β1 is able to inhibit proliferation, and cells whose response to TGF-β1 is reduced contribute to malignant transformation. The deletion of p16, Rb and the increase of Cyciln D1 contribute to the occurrence and development of ovarian cancer. The relative decrease of AP% in malignant tumors is of great significance for malignant tumor. The negative correlation between bcl-2 and p16 suggests an interaction between apoptosis and proliferation. Due to the interaction between apoptosis-related proteins and cell cycle regulators, malignant tumors maintain a high cell accumulation rate.