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目的 探讨人精子细胞膜结合型透明质酸酶 (PH 2 0 )基因对乳腺癌细胞MDA4 35在体内外生长的影响。方法 将PH 2 0cDNA转染到人乳腺癌细胞株MDA4 35中 ,形成MDA4 35 PH2 0。将MDA4 35 PH2 0种植在软琼脂培养皿中 ,以转染空载体pcD NA3的MDA4 35细胞为对照 ,观察PH2 0基因对乳腺癌细胞集落形成能力的影响。再将MDA4 35 PH2 0和MDA4 35 pcDNA3分别以同等条件接种到 5周龄裸鼠皮下 ,在不同时间点上比较两组鼠身上肿瘤大小。结果 转PH2 0基因的MDA4 35细胞在软琼脂培养基中集落形成数量明显高于对照组 ,同时MDA4 35 PH2 0在裸鼠身上的肿瘤生长速度也显著高于对照组。结论 PH 2 0使乳腺癌细胞MDA4 35的恶性度增高并促进其在体内的生长能力。
Objective To investigate the effect of human sperm cell membrane-bound hyaluronidase (PH 2 0) gene on the growth of breast cancer cell MDA4 35 in vitro and in vivo. Methods PH 2 0 cDNA was transfected into human breast cancer cell line MDA4 35 to form MDA4 35 PH20. MDA4 35 PH2 0 was planted in soft agar plates, and the effect of PH2 0 on colony-forming ability of breast cancer cells was observed using MDA435 cells transfected with empty vector pcD NA3 as a control. Then MDA4 35 PH2 0 and MDA4 35 pcDNA3 were inoculated subcutaneously into 5-week-old nude mice under the same conditions, and the tumor size was compared between the two groups at different time points. Results The number of colonies of MDA4 35 cells transfected with PH20 gene in soft agar medium was significantly higher than that in control group. Meanwhile, the growth rate of MDA4 35 PH20 in nude mice was also significantly higher than that in control group. Conclusion PH 2 0 makes breast cancer cell MDA4 35 more malignant and promotes its growth in vivo.